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Samples of GST-ShHTL1 and GST-AtHTL were also subjected to western blotting after separation by SDS-PAGE
GST enzymes from various sources, both native and recombinantly expressed as fusion to the N-terminus of target proteins, are easily purified using glutathione (GSH) agarose beads is well documented and provides a one-step, high purity affinity purification
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Elife 8 , e49673 (2019)
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A modest reduction in triagonist-induced insulin secretion was observed in Gcgr / islets (Fig
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