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In essence, 30 min before anaesthesia, mice received a single intraperitoneal dose of a ferrostatin (Fer-1 (10 mg per kg at 200 l), UAMC-2303 (200 l 2.5 mM solution in 0.9% NaCl), or 2OH-oestradiol (10 mg per kg, 200 l) or a corresponding vehicle control as indicated
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The calculator recognizes that human precision is limited by syringe markings and solves the common frustration with peptide reconstitution you never have to guess whether 1.33 mL or 2.5 mL of BAC water is the "correct" starting amount, or work out how many syringe units your dose comes to often a hard-to-read number like 13 or 22
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