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Briefly, 96-well ELISA microplates were coated with HGFR/c-MET Fc chimera (50 ng/well), blocked with 1% BSA, 5% sucrose, and 0.05% sodium azide in PBS, and then incubated for 2 h at 37C with HGF samples
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The amount of bac water to use directly affects how strong the solution will bealso called concentrationand that matters when dosing experiments in cell cultures or test animals
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The reaction was carried out at 25 C for 24 h
