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Quantitative real-time polymerase chain reaction (qRT-PCR) In accordance with previous studies [36], we identified 10 mRNAs for real-time fluorescent quantitative PCR (qRT-PCR) verification (Additional file 1)
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Tirzepatide Efficacy vs
Plasma peptide concentrations for each blood compartment and time point in the experiment were measured using commercial ELISA kits (for exendin-4: Creative Diagnostics Exendin-4 ELISA kit (cat
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