HepG2, HT1080, and MEF cells were cultured in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (P/S) at 37 C with 5% CO 2
For an insulin syringe (U-100 scale, 100 units = 1 mL): For published preclinical context: mouse studies (Billon 2023, PMID 37739806) used 25 mg/kg IP twice daily a much higher per-kilogram dose than community injectable users typically describe
So, the skin not only suffers intrinsic aging, which is common to all organs, but it also suffers extrinsic aging
doi:10.1073/pnas.0707258104
Different forms exist (capsule, sublingual, solution), but no official dosing guidelines are established due to limited studies
Of course, 2L is a lot, and a serving of Liquid Gold isn't anything close to equivalent, but the hazard is worth knowing