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Reconstituted lyophilized tirzepatide typically needs to be used within 28 to 30 days when kept in the refrigerator at 2 to 8 degrees Celsius

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For cell number measurements in 24-well plates, unstained plates were scanned in bright field using SparkCyto (Tecan) at 37 C under 5% CO 2

Store lyophilized vials in the original amber glass or dark packaging, away from any direct or indirect light source including natural window light