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Following a washing step, Alexa Fluor 594-conjugated goat anti-rabbit IgG (1:400, Molecular Probes, Waltham, MA, USA) and/or Alexa Fluor 488-conjugated goat anti-mouse IgG (1:400, Molecular Probes) were/was added as the secondary antibody and DAPI was used for nuclear staining

One study from 2006 found that CJC-1295 successfully elevated GH levels for longer than normal and was generally well tolerated, but more research is needed to say for sure

Vitamin C: intravenous use by complementary and alternative medicine practitioners and adverse effects. PloS one vol

10.1016/s0076-6879(85)13050-8 Methods Enzymol

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