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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions
Unlike compounds that rely heavily on hormonal pathways, the BPC-157 Peptide operates primarily through the upregulation of growth factors and the modulation of the nitric oxide (NO) system
The methylation of DNA is particularly important, as it helps to regulate gene expression and maintain the integrity of the genome
Experimental Reagents and materials Bovine serum albumin (96%, BSA, Sigma) was purchased in lyophilized-powder form and used without further purification
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