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A centrifuge was used at 13,000 rpm for 35 min at 4 C to remove cellular debris
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Since heavy metal exposure elevates ROS (Jaishankar et al., 2014), one intriguing possibility is the heavy metal content of the lunar regolith simulant (Long-Fox et al., 2023) causes increased genome oxidation, which in turn leads to telomere shortening
doi: 10.1016/j.conb.2004.06.003 50 FingerTEBarlowLA
Annu Rev Pharmacol Toxicol 29: 365402
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