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Tryptophan oxidation is the degradation route most commonly detected by RP-HPLC in stored melanocortin analogues, with tyrosine at position 2 and histidine at position 6 secondary sites of concern, and freeze-thaw cycling accelerates both aggregation and oxidative degradation

Figure 2 Structure of different PLC isoforms In mammals, 13 different PLC isozymes have been reported and are divided into six different subfamilies, namely , , , , , and (Suh et al., 2008