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B.YesilyurtF.DemirdogenF.AtesD.et al (2024)

Because Jardiance and diuretics can increase urination, taking these medications together may lead to low fluid levels in the body, leading to dehydration and potential kidney problems

Subsequently, the brains were collected and postfixed in paraformaldehyde for 48 h at 4 C and sectioned to obtain 12-m slices, which were permeabilized with 0.05% Triton X-100 (Vetec, Speyer am Rhein, Germany) solution in PBS for 30 min and subsequently incubated with blocking solution containing 5% bovine serum albumin (SigmaAldrich), 2.5% normal goat serum (Thermo Fisher Scientific, Waltham, MA), and 0.02% Triton X-100 diluted in PBS for 1 h

Thanks to the large bathochromic shift accompanying the reaction, the peak of the excess of the derivatization reagent does not appear on the chromatogram 32

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L.KalowW.et al (1991)