Brown TJ, Sedhom R, Gupta A
Rizvi S, Raza ST, Ahmed F, Ahmad A, Abbas S, Mahdi F
Absorbance (418 nm) was assessed using a plate reader set at 37 C with a total reading time of 4560 min and 15 min intervals between reads

Introduction The technology of semen cryopreservation effectively promotes the application of technologies including genetic resource conservation, zootechnical management, and artificial insemination ( However, the cold shock and alterations in atmospheric oxygen seriously led to biochemical, biophysical, ultrastructural, molecular, and functional alterations ( To ameliorate oxidative stress-related damage, the supplementations of antioxidants, including vitamins, coenzymes, exosomes, liposomes, nanomaterials and trace elements such as selenium (Se), copper, and zinc to semen extender have been widely conducted ( According to a previous study, the co-supplementation of vitamin E effectively ameliorates the low toxicity threshold of Se ( GSH, an endogenous antioxidant synthesized from different amino acids including glycine, cysteine, and glutamate, effectively inhibits oxidative stress-related damage and altered ROS production level (33)

Oxidative stress is implicated in the pathogenesis of SLE through multiple mechanisms, including mitochondrial dysfunction, activation of the type I IFN pathway, and the generation of oxidative DNA damage [130]
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