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After centrifugation at 1000 x g for 10 min, the supernatant (S1) was centrifuged for 20 min at 17.500 x g to yield the supernatant S2 and the pellet P2 (crude synaptosomal fraction)
BPC 157 was given per-orally, in drinking water (10 g/kg, 10 ng/kg, i.e., 0.16 g/ml, 0.16 ng/ml, and 12 ml/rat/day) till sacrifice, or alternatively, 10 g/kg and 10 ng/kg intraperitoneally
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Extend planned duration (8 weeks becoming 12-16 weeks)
Chromatographic conditions employ a C18 reversed-phase column, with acetonitrilewater solution (adjusted to an appropriate acidity with phosphoric acid) as the mobile phase
For women, this raises additional concerns about exposure during sensitive life stages (e.g., pregnancy, postpartum, perimenopause)
