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Insulin: Glutathione could potentially interact with insulin, leading to fluctuations in blood sugar levels

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A total 2 10 5 cells were used in each experiment for detection of total glutathione and 10 6 cells for GSSG measurement

Co-cultured cells were collected in one 15 ml falcon tube and lysed by RIPA buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS) containing protease inhibitor cocktail (Sigma-Aldrich) and PMSF (Sigma-Aldrich) with 1 M Sodium-Orthovanadate pH 10(Sigma-Aldrich)

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