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For Caspase-3 staining, the tissues were blocked with 10% normal horse serum for 30 min then incubated with Caspase-3 antibody (1:300, BD Biosciences, Macquarie Park, NSW, Australia) overnight, followed by secondary antibodies (Goat anti-rabbit HRP, 1:200, Vector Laboratories, Burlingame, CA, USA) for 45 min

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After reconstitution with bacteriostatic water: store at 4C, use within 2128 days

Submit required medical documentation: Your provider will need to submit supporting medical documentation to demonstrate why the GLP-1 medication is necessary for your treatment

Accurate patient, medicine, and clinician information helps prevent delays caused by mismatched names, old directions, or incomplete quantities

middle panel) and VDAC (lane 2