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Ice crystal formation damages peptide structure irreversibly
Wean J, Kowalsky AH, Laker R, et al
The plate containing the frozen cells were lyzed with Pierce RIPA Lysis and Extraction Buffer (Thermo Fischer Scientific) supplemented with 1 cOmplete Protease Inhibitor Cocktail (Roche) (220 L/well) for 10 min on ice on a tilting board
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In many patients, it is a metabolic and cognitive risk amplifier
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