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To take into consideration the compositional nature of the data, all DESeq2-based results were complemented with graphical representations of abundance log-ratio (between finish and start matched samples) rankings

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Signs of bacterial contamination: Cloudiness (should be clear) Particles or floating material Unusual odor Color change Physical degradation signs Visual changes indicating degradation: Clear solution becomes cloudy Color change (especially copper peptides) Precipitation or crystals forming Separation of layers When in doubt: Discard