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Global protein abundance profiling by mass spectrometry Sample preparation (A) Flash frozen liver sections (~25 mg) from WT or Gclc / mice (3 biological replicates each per TMT sample) were thawed on ice and resuspended in DPBS, supplemented with protease (cOmplete, EDTA-free protease inhibitor cocktail, Roche, #11873580001) and phosphatase (PhosSTOP, Roche, #4906845001) inhibitor tablets

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Cancer cells often maintain intracellular GSH at 110 mM up to 1000 times greater than extracellular levels, which range from ~ 2 to 20 M [8]

FOXO4-DRI induces keloid senescent fibroblast apoptosis by promoting nuclear exclusion of upregulated p53-serine 15 phosphorylation