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After several hours, its still fine in water

Materials and methods Reagents Bovine insulin was obtained from Sigma-Aldrich (St Louis, MO, USA) and dissolved in acidic water (pH 23) to make a stock solution

A pure RBC suspension can reliably be achieved by density-gradient centrifugation (2500 RPM, 15 min, RT)

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The individual diagnoses were assigned according to the International Classification of disease 10 (ICD-10)