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Human hepatoma Hep-G2 cells (ATCC, Cat #HB-8065) were cultured in EMEM containing 2 mM l-glutamine, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate (Gibco), fortified with 10% syringe-filtered FBS (Gibco), 1% penicillin/streptomycin (Gibco), and 0.2% Normocin (InvivoGen)