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Glutathione supplementation can also be considered in resistant or recurrent melasma as part of adjunctive therapy

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(Basically following Dr Burrasconos treatment protocol of the time)

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The absorbance of this azo chromophore was then detected photometrically at 540 nm using a BioTek Instruments Powerwave 340 microplate reader (Winooski, Vermont), and unknown sample values were determined using a nitrite standard curve, via software (BioTek Gen5)