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Spiking experiments involved addition of known amounts of pure NAD and glutathione compounds individually at different stages of the extraction (Supplementary Table 1), followed by quantification using colorimetric assays

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Over three months, glutathione supplementation resulted in improved liver enzymes, lower triglycerides, and lower HbA1c, a blood sugar level marker

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If the phenotypes of the mutants can be successfully restored to wild type, this will confirm that only the correct genes have been deleted and further validate the specific phenotypes ascribed to each GDH mutant