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J Appl Physiol 60 : 532538 Reed DJ, Babson JR, Beatty PW, Brodie AE, Ellis WW, Potter DW 1980 High-performance liquid chromatography analysis of nanomole levels of glutathione, glutathione disulfide, and related thiols and disulfides

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The cells were subsequently treated with DCFH-DA (10 M, Bio-lab) for 1 h in the dark, and washed three times with PBS

This molecule is essential because it is involved in hundreds of chemical reactions

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