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The disulfide bond between Pt GSTF8 residue C13 and GSH was reduced on-column with 20 column volumes of rPPS buffer (20 mM potassium phosphate, 200 mM NaCl, 10 mM DTT, pH 7.4), at a flow rate of 5 ml min 1 and the column was then washed with 40 column volumes of PPS buffer at a flow rate of 5 ml min 1 to remove free GSH

This domain has a similar composition to thioredoxins, which act as antioxidants by facilitating the reduction of other proteins

Ageing, sun exposure, pollution, smoking, stress, lack of sleep, and poor nutrition can slowly drain the bodys natural antioxidants

The key question isn't whether it works in the short term, but whether it remains safe and beneficial when used long-term

Toxins (Basel) (2017) 9(4):132

& Appling, D