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After washing three times with PBS, the cells were incubated with secondary antibodies conjugated to Alexa Fluor 488 or 594 for 1 h at room temperature in the dark
Summary: B12 deficiency after gastric sleeve surgery occurs because the procedure reduces intrinsic factor production and gastric acid secretion, impairing vitamin B12 absorption and requiring lifelong supplementation
Summary: GLP-1 receptor agonist fatigue management strategies include optimising nutrient-dense food intake, maintaining proper hydration, gradual dose titration, managing gastrointestinal symptoms, regular physical activity, and seeking medical review for persistent or severe tiredness
For cardiovascular risk reduction, it is only approved for adults 18 years and older with type 2 diabetes
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