Meanwhile, immunofluorescence staining showed that 4-HNE was mainly detected in SP-C + cells, rather than EMBP + or LY6G + cells, indicating aberrant lipid peroxidation in AT II cells (Figs
Briefly, frozen livers were allowed to thaw to room temperature and homogenized by macerating with a disposable glass pipet
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The intracellular glutamate concentration is nearly 10-fold higher than the Km value, but intracellular cysteine concentrations approximate the apparent Km value [10] , indicating that cysteine may be a limiting substrate for GCL
Ding X, Saxena NK, Lin S, Gupta NA, Anania FA
This amount helps maintain glutathione in its active, "reduced" state and prevents it from being depleted too quickly by oxidative stress