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Protocol researcher Compound arrived lyophilized, correctly labeled, with a batch-specific CoA
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The culture medium, referred to as FAX, consisted of N2B27 supplemented with bFGF (15 ng/ml, PeproTech), Activin A (20 ng/ml, PeproTech), and XAV939 (1 M, Selleck).The N2B27 medium was prepared by combining equal parts of DMEM/F12 and Neurobasal (both from GIBCO), with the following additives: 0.5% N2, 1% B27, 1% GlutaMAX, 1% non-essential amino acids (NEAA), and 0.1 mM -mercaptoethanol (all from GIBCO).For passaging, EpiSCs were dissociated using Accutase (Sigma) and seeded as single cells in FAX medium supplemented with 5 M Y27632 (Selleck)
