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DSB detection was based on expression levels of -H2AX, a classic biomarker for DSBs, which could be visualized by immunofluorescence microscopy

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The reaction mixture, consisting of 1 g RNA template, 1 l iScript Reverse Transcriptase, 4 l 5 iScript Reaction Mix, and Nuclease-free water, was subjected to priming at 25 C, reverse transcription at 46 C, and inactivation at 95 C