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Wang H, Liu C, Zhao Y, Gao G

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Plasma which was not deproteinized prior to storage was removed from storage (80 o C and 4 o C, respectively) and mixed with an equal volume of cold 5% SSA and incubated for 10 min at 4 o C (on ice)

Both in vitro and in vivo treatment significantly inhibited TLR-4 and elucidated to the role of estrogen receptor 1 (ESR1)/ NLR family, pyrin domain-containing protein 3 (NLRP3)/CASP-1 signaling [118]