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RNA isolation and real-time quantitative PCR Total RNA was isolated with E.Z.N.A. Total RNA Kit I (Omega Cat#R6834-01) and reverse transcription (RT) reactions were performed with High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Cat#4368814) according to manufacturers protocol

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The FDAs formal guidance against compounded semaglutide projected to eliminate USD 3.6 billion in compounding pharmacy and telehealth revenue in 20252026 creates disruption risk for approximately 1.5 million patients receiving compounded GLP-1 outside branded channels

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