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Following subcutaneous administration in clinical models: Both components demonstrate prolonged absorption with peak plasma concentrations occurring 24-72 hours post-injection GLP3 exhibits approximately 6-day half-life enabling once-weekly dosing Cagrilintide demonstrates 120-165 hour half-life (5-7 days) suitable for weekly administration Lipid conjugation of both peptides enables albumin binding and extended systemic circulation Distribution patterns show systemic exposure with concentration in metabolically active tissues The fatty diacid modifications on both peptides (C20 for GLP3 , eicosanedioic acid for cagrilintide) serve as albumin-binding moieties that dramatically extend plasma residence time compared to native peptides

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Mol Cell Proteom 20:1000022 Neuzil J, Dyason JC, Freeman R, Dong L-F, Prochazka L, Wang X-F, Scheffler I, Ralph SJ (2007) Mitocans as anti-cancer agents targeting mitochondria: lessons from studies with vitamin E analogues, inhibitors of complex II

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Finally, the sections were washed in PBS, mounted onto poly-lysine-coated glass slides, cover-slipped using fluorescence mounting medium and visualized via an Olympus FV1000 confocal microscope (Olympus, Shinjuku, Tokyo, Japan)