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Recordings of brain swelling were performed in rats before sacrifice after complete calvariectomy was performed ( Rats were laparatomized before sacrifice for the corresponding presentation of the peripheral vessels (azygos vein, superior mesenteric vein, portal vein, inferior caval vein, and abdominal aorta)

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Reconstitution must be performed by trained personnel in an appropriate environment (e.g., a laminar flow cabinet or isolator where required by the protocol)