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On day 6 of differentiation, the medium was changed to glucose/phenol red/glutamine/pyruvate-free DMEM (Thermo Fisher Scientific) supplemented with 0.5% FCS, 4.5 g l 1 glucose, 4 mM glutamine, 0.5 mM pyruvate and 5 g ml 1 insulin

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The lack of solid clinical evidence on long-term safety, especially for ongoing use, creates uncertainty about potential interactions

To view a copy of this licence, visit About this article Cite this article Zhong, Y., Chen, J., Shi, N