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That said, it's worth knowing the difference between typical irritation and something that needs prompt attention
Liver samples were then mixed with 4X Laemmli sample buffer (8% SDS, 40% glycerol, 0.4% bromophenol blue, 240 mM Tris, pH 6.8) with freshly added -mercaptoethanol and were heated for 5 mins at 95 C
Common side effects include nausea, vomiting, diarrhea or constipation, indigestion, stomach pain, and a decrease in appetite
Anything beyond that starts the degradation clock