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This is particularly prevalent in the United States, where patients across various stages of life may receive care from multiple providers, making it difficult to track medical records or assess cumulative medication burdens

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Then cDNA was synthesized using SuperScript TM IV VILO TM Master Mix (Invitrogen, Waltham, MA, United States) and diluted 101 by DNase/RNase-Free Distilled Water (Invitrogen)