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Secondary analysis using an ELISA based approach confirms GPx3 is substantially reduced in children affected with konzo To validate the findings obtained using proteomic-based applications, we sought to determine if the reduced abundance of GPx3 protein in konzo affected children as compared to their unaffected siblings could also be detected using an orthogonal ELISA-based approach

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Clinical practitioners often pair this method with PRP or exosome therapy for compounded results

(C and D) Structural models of the catalytic site of Cu(II)-free tyrosinase, in which the side chain of the His54 residue takes conformations orienting toward the CuA-binding site and the surface of the caddie protein, respectively