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113 The impact of CR on cancer is also interconnected with mutations and oncogenic pathways

Our results have many similarities when we compare the results of a large meta-analysis carried out by JimnezFernndez, 2020 42

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At 48 h post-transfection, cells were harvested in ice-cold PBS using Trypsin-EDTA (Lonza, Switzerland), centrifuged at 1000 g for 3 min, and washed with ice-cold PBS

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This means higher IGF-1 levels increase risk for certain cancers and make some more difficult to treat