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Maintenance Dosing and Long-Term Use Once you reach your target maintenance dose, typically 2.0 to 2.4 mg, you continue that same dose every seven days indefinitely as long as you remain in treatment

Then, incubated at 37 C under anaerobic conditions (80% N 2 , 10% CO 2 , and 10% H 2 ) for 0, 6, 12, and 24 h