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HFpEF, HFmrEF or HFrEF, respectively), we focus the review on HFpEF and HFrEF

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The long answer is a bit more scientific

After harvesting the cells by centrifugation at 4,000 g for 30 min, the cells were resuspended in lysis buffer (50 mM sodium phosphate, pH 8.0, 300 mM NaCl, 10 mM imidazole) and lysed using a sonication for 1 h with a 10-s pulse mode (10 s on and 10 s off) on ice

Purified recombinant protein (3 g) was resolved using 18% w/v SDS-PAGE in reduced (+-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250

Forecasting obesity and type 2 diabetes incidence and burden: The ViLA-obesity simulation model