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Slopes for inhibitor samples and enzyme control were calculated by dividing the net RFU (RFU 2 RFU 1 ) values by T ( T 2 T 1 )

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The result, expressed in EU/mg, appears on the Certificate of Analysis at cell-culture-reference acceptance limits, sized so the material can be used in sterile pituitary-cell or signalling assays without LPS interference

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