Following primary antibody incubation, cells were treated with goat anti-rabbit IgG H&L Alexa Fluor 488 (ab150077, Abcam), donkey anti-goat IgG H&L Alexa Fluor 647 (ab150131, Abcam) and DAPI (Invitrogen) for 1 hour in the dark at room temperature
For participants 1 and 2, the bipolar reference montage was adjusted during early recording period and the stimulation safety testing period
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4 Discussion and conclusions Cells respond to nutrient fluctuations by adjusting anabolic versus catabolic processes