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A 20 l mix, containing 8 l of cDNA sample, 10 l of SYBR Green PCR Master Mix (Thermo Fisher Scientific), and 2 l of 10 mM forward primer and reverse primer mixture was used to performed Quantitative RT-PCR with a StepOne Plus Real-time PCR system (Applied Biosystems, Foster City, CA, United States)

Published records associate BPC-157 with the peptide sequence GEPPPGKPADDAGLV and a defined molecular formula, which can support compound characterization and documentation review [1] [2]

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