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Butler AE, Janson J, Bonner-Weir S, Ritzel R, Rizza RA, Butler PC

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After incubation for 15 min at 30 C, 1,000 rpm using a 3 mm shaking diameter, cell debris was removed by centrifugation (4,400 g at 4 C for 30 min) and the supernatants were analysed by HPLCMS at two different dilutions (undiluted and four times diluted with 67% methanol, 0.2% hydrochloric acid in H 2 O)

The authors noted that the damaging effects of homocysteine begin to appear between 8 and 10 mol/L.36 Hyperhomocysteinemia may increase the risk of Alzheimers disease by compromising the integrity of the bloodbrain barrier, allowing toxic substances to pass through to brain tissue

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