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All mice received 2 days of 10 mL/kg body weight sterile saline subcutaneous injections before beginning the treatment phase to allow for acclimation to the handling and stress associated with injections

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Adding micronutrient supplementation to address any co-factors needed for B12 activation

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Key takeaways B12 shots help most when theres a true deficiency or an absorption problem (like pernicious anemia or after GI surgery)