Human Embryonic Stem Cell Culture (HES) HES2 cells (ES International, Singapore) were cultured on irradiated feeders in Knock-out DMEM supplemented with 20% Knock-out Serum Replacement, 2 mmol/L glutamine, 1% non-essential amino acids (NEAA), 100 U/mL penicillin, and 100 g/mL streptomycin (all Thermo Scientific) and further supplemented with 10 ng/mL FGF2 (PeproTech)
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5a, b, while neither the Arr nor the W mutants exhibited any differences
10.3174/ajnr.a2637 Am
A., Verhagen, J
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