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Minimize freeze-thaw cycles, which progressively degrade peptide integrity
Briefly, cells were incubated in a serum-free Dulbeccos modified Eagles medium (DMEM) containing 10 M DCFH-DA at 37 C for 30 min
A 1 mg dose then equals 0.04 mL, or 4 units on a U-100 insulin syringe
Robust trials showing injected MIC causes independent fat loss are lacking
10.1016/j.freeradbiomed.2022.05.003 20 FlorianS.WinglerK.SchmehlK.JacobaschG.KreuzerO
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