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They explored the correlation between telomere length and mtDNA copy number in bovine embryos and treated them with telomerase inhibitors

This process, along with GCL inhibition and the formation of mixed disulfides between GSH and protein sulfhydryl, concurred to a steady-state decrement of free GSH concentration and, consequently, to an intracellular redox state shift toward more oxidizing conditions, which could be required for avoiding fast reduction of oxidized cysteines allowing the upstream ROS-induced autophagic stimulus to be propagated

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Bacterial counting assay: Gradient dilution method was employed to count living bacteria in solution or tissue (tissue was homogenized in 10 mL PBS to get a bacterial suspension)