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For extended periods, storage at 20 C is recommended
Townsend et al., 2003
If the pattern fits early adaptation, a short period of monitoring often makes sense
The effects are usually minimal
It is also a precursor of guanidophosphate, phosphoarginine and creatine, the high energy compounds found in muscles
Total RNA was then reverse transcribed and real-time PCR with HiScript IV All-in-One Ultra RT SuperMix for qPCR (R433-01, Vazyme) was performed on the qTOWER (analytikjena) real-time PCR instrument
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