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Live-cell z -stack and time-lapse imaging were performed using an Olympus FV3000RS confocal microscope (Olympus) equipped with a stage-top incubator at 37 C, 5% CO 2 and 100% humidity
Furthermore, AKG can decrease protein degradation in muscles, and plays a protective role in the gut
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By mitigating oxidative damage in animals exposed to captivity stress or environmental toxins, antioxidants have been used to improve breeding success rates, exemplified by their use in endangered amphibian programs