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The MSIA tips are then rinsed with a series of buffer and water rinses to remove salts and detergents ( Figure 1 , step 3), after which captured IGF-1/LR3 are eluted from the MSIA tips by iteratively pipetting an elution solvent of 33% acetonitrile in 0.4% trifluoroacetic acid, TFA (step 4)
The optimizations and full runs of the multiplex panel were executed using the seqIF methodology integrated into the Lunaphore COMET platform (characterization 2 and 3 protocols, and seqIF protocols, respectively) 97
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